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• Available in both chemically competent and electrocompetent formats

• Readily accepts large plasmids due to deoR mutation

• Excellent for subcloning and library construction

• Efficient blue/white screening

PEAK™ Efficiency competent cells are appropriate for routine subcloning and cDNA library construction. In addition to supporting blue/white screening, the cells provide recAI and endAI mutations to improve plasmid DNA quality and minimize recombination. Includes pUC19 control plasmid for monitoring transformation efficiencies.

Subcloning Efficiency: > 107 cfu/µg pUC19

Library Efficiency: > 109 cfu/µg pUC19 in convenient 100µl aliquots

Genotype: deoR, endA1, recA1, relA1, gyrA96, hsdR17(rk - mk +), supE44,

thi-1, delta(lacZYA-argFV169), f80dlacZdeltaM15,F -,Y -

Recommended storage: -70°C

PEAK is a trademark of Bioline USA, Inc. DH5a is a trademark of Invitrogen Corporation.