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Back to   Home   Electrophoresis & Blotting   Acrylamide   SPRINT NEXT GEL Protein Electrophoresis System
SPRINT NEXT GEL Protein Electrophoresis System
SDS-PAGE on SPRINT NEXT GEL. 10cm x 10cm x 0.75mm 10% acylamide gel run at 300 volts for 30 minutes. Lane A: AMRESCO Mid/Low Range Protein Molecular Weight Marker (Code: J450). Lane B Total E. coli lysate (50µg).
SDS-PAGE on SPRINT NEXT GEL. 10cm x 10cm x 0.75mm 10% acylamide gel run at 300 volts for 30 minutes. Lane A: AMRESCO Mid/Low Range Protein Molecular Weight Marker (Code: J450). Lane B Total E. coli lysate (50µg).
SDS-PAGE on SPRINT NEXT GEL. 10cm x 10cm x...
Western blot of Rat-l fibroblast lysates- Courtesy of Dr. June Yun at Northeastern Ohio Universities College of Medicine (NEOUCOM). Identical lysates were run on 10% SPRINT NEXT GEL or 10% Laemmli gels, transferred onto PVDF membranes and incubated with anti-GAPDH antibodies. Lane A: 30µg lysate. Land D: 50µg lysate. Lane E: 10µg lysate. Procedures, including gel casting, running, transfer to membrane, and blot development, with SPRINT NEXT GEL were performed in a single day.
Western blot of Rat-l fibroblast lysates-...
 

Do an entire western blot in one day—from gel casting through blot development

• Fast—Cast and polymerize a 10cm x 10cm x 0.75mm mini-gel in less than 15 minutes. Gel running time is less than 30 minutes.
• Convenient—No stacking gel is required
• Cost savings—No hazardous shipping charges
• Versatile—Compatible with standard staining methods and downstream applications including western blotting, 2-D electrophoresis, sequencing, MALDI analysis, and LC-MS

SPRINT NEXT GEL™ enables researchers to cast, polymerize, and run a mini-gel in less than 45 minutes. Provided as a novel, ready-to-pour SDS polyacrylamide solution, it polymerizes into a unique support matrix for the electrophoretic separation of denatured proteins. The proprietary chemistry of SPRINT NEXT GEL eliminates the need for a stacking gel while providing sharp band resolution over a wide molecular weight range. Kits are available at two different acrylamide concentrations and require only the addition of APS and TEMED to initiate polymerization prior to pouring the gel.

Includes: NEXT GEL™ Running Buffer, 20X. Each 7.5ml will prepare a 10cm x 10cm x 0.75mm mini-gel

Item:    Description   Size       Price Qty  
M312-100ML     SPRINT NEXT GEL, 10% Solution*   100ml       $  40.30  
M312-500ML     SPRINT NEXT GEL, 10% Solution   500ml       $  149.80  
* Separation Range: 10kDa-200kDa  
M311-100ML     SPRINT NEXT GEL, 12.5% Solution   100ml       $  40.30  
M311-500ML     SPRINT NEXT GEL, 12.5% Solution   500ml       $  149.80  
** Separation Range: 3.5kDa-100kDa  
 

 

 

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