Phenol : chloroform : iso-amyl alcohol (25:24:1) (v/v/v), low pH for biotechnology

Supplier: VWR

Danger
0966-100ML 0966-400ML
97064-710 97064-712
Phenol : chloroform : iso-amyl alcohol (25:24:1) (v/v/v), low pH for biotechnology
Phenol : Chloroform : iso-Amyl alcohol
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.

  • Manufactured Using Exceptionally High Purity Phenol that is Double Distilled & Packaged Under Nitrogen
  • Liquid Phenols Offer a Safer, Easier Alternative to Crystalline Phenol
  • Optimized pH for the Purification of RNA
  • Long Term Stability > One Year


MDL Number: MFCD00133763
CAS Number: 136112-00-0
UN: 2810
ADR: 6.1,II
Specification Test Results
O.D.@ 330nm = 0.2
O.D.@ 405nm = 0.1
O.D.@ 510nm = 0.1
pH @25°C 4.9 - 5.5
Purity = 99 %

 

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