Keep my session open?
Ending In 
The session is expired
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

VWR Life Science Yeast Genomic DNA Purification Kit

Supplier: VWR

VWRV1B1586KIT VWRV1B1586KIT50RXN 1B1586KIT50RXN 1B1586-KIT-200RXN
Yeast Genomic DNA Purification Kit yields high molecular weight DNA from yeast using a non-enzymatic lysis buffer and no glass beads

  • Rapid purification yields high quality, high molecular weight DNA
  • No enzymes, glass beads or freeze thawing for cell lysis
  • Method is phenol, chloroform and column-free
  • Easily scale purification as needed

The protocol is simple, requiring about 1 hour to complete, and may be scaled for large or small samples. The purification process begins with lysis of the yeast cell wall with optional RNase A treatment, and is followed by protein precipitation and then DNA precipitation. Unlike many conventional yeast genomic DNA purification methods, no phenol, chloroform or columns are required. The kit, which includes Lysis Buffer, Protein Precipitation Solution, RNase A, 10 mg/ml and Resuspension Buffer, works with liquid cultures as well as colonies. The purified DNA yields vary for different yeast strains and is also dependent on cell density. The kit is optimized for Saccharomyces cerevisiae, with an average yield of 1.5 µg DNA per 5 x 107 cells as measured by VWR’s Fluorescent DNA Quantitation Kit. High quality DNA obtained with the Yeast Genomic DNA Purification Kit is compatible downstream applications, such as, PCR, restriction enzyme digestions and Southern blotting.

Ordering information: Kit includes Yeast Lysis Solution; Ribonuclease A (RNase A); Protein Precipitation Solution; TE, pH 8.0.


 

ORDER

Expand All / Collapse All
Description No. of Reactions Supplier No. Catalog Number Unit List Price Quantity
Yeast Genomic DNA Purification Kit 50 VWRV1B1586KIT50RXN 1B1586.KIT.50RXN Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted You must log in to order restricted items. We request that you provide the required business documentation to purchase this product for the first time.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email info@bioexpress.com or fax 800-574-7892 referencing your BioExpress account number. Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
BioExpress will not lift restrictions for residential shipping addresses.

Enter Certificate Details

Enter the Lot number:

Choose from recent batch/lot numbers:

Or
Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected.To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty
 

Terms & Conditions

Prices:
Prices shown are current at time of publication. Where changes have occurred, prices prevailing at the time of shipment will apply. Prices are subject to change without notice.

Quotations:
Our sales and customer service departments welcome quotations for laboratory set-ups and multiple-unit orders. Your inquiries are welcome and appreciated.

Payment Terms:
Payment terms are Net 30 Days on approved credit, F.O.B. warehouse or point of manufacture. Shipping charges are prepaid and added to the invoice. C.O.D. orders will be charged a $10.00 handling fee plus shipping charges. AMEX, MasterCard, and Visa are accepted.

Minimum Order:
There is no minimum order at BioExpress. Full case quantities or minimum quantities may apply to non-stock or specialty items.

DELIVERY & SHIPPING
All orders are shipped F.O.B. warehouse or point of manufacture. Shipping charges are prepaid and added to the invoice. Orders are usually shipped within 24 hours of receipt. We ship by the most efficient and economical means available. Extra charges apply to HAZMAT and dry ice shipments.

RETURN GOODS POLICY
If you would like to return an item, please call BioExpress customer service or contact your local representative for an RGA number. All returns must include an RGA number, include original packaging and be shipped prepaid. Items older than 30 days, sterile, or special order items are subject to review. A 15% restocking fee applies.

WARRANTIES AND LIABILITIES
BioExpress accepts no responsibility and shall not be held liable for loss, damage, expense, consequential, or accidental damage. This includes the damage to property, person, or premises resulting from these products. BioExpress MAKES NO WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING, WITHOUT LIMITATION, THE WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURCHASE. BioExpress expressly disclaims all other warranties, whether express, implied or statutory, including the warranty of MERCHANTABILITY AND FITNESS FOR USE. In no event will BioExpress be liable for consequential damages arising from the use of products described in this catalog.

Print These Terms