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Fast-Ion Midi Plasmdi Kits, IBI Scientific


IBI’s Midi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA/RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate binds to the column. The contaminants are washed away with a wash buffer. The purified plasmid DNA is eluted by a high salt buffer and precipitated with isopropanol. The entire procedure can be completed in less than 2 hours.

The plasmid DNA is suitable for transfection, sequencing reactions, PCR, microinjection, restriction digestion, and in-vitro transcription.



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Description Kit Size Supplier No. Catalog Number Unit List Price Quantity
Midi Fast-Ion Plasmid Kit 25 Preps IB47111 G-1978-1 Each Retrieving Restricted
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Type Ion-Exchange Resin Column
Sample Size 50 mL High Copy Plasmid/ 100 mL Low Copy Plasmid
Purity Equal to that obtained by 2X CsCl-Gradient Centrifugation
Binding Capacity 500 µg
Operation Time 120 min or less
Operating Type Gravity Flow
Yield Up to 200 µg of Plasmid DNA
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