, Direct Blue 14
Trypan blue 0.4% in aqueous solution Ready-to-Use, sterile
A pre-mixed dye solution used in cell culture applications to determine cell viability. A researcher can remove a sample of cells from culture and combine in a 1:1 ratio with the Trypan Blue solution. Under the microscope, dead cells will appear a blue color and viable cells will appear clear and translucent. Using a hemacytometer (gridded microscope slide), a researcher can quantify the percentage of dead cells within a population. Stained cells are ready for counting within five minutes.