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ICT Accutase Cell Detachment Solution

Supplier: INNOVATIVE CELL TECHNOLOGIES BE

AT104
490007-741
ICT Accutase Cell Detachment Solution
Enzymes
Accutase can be used whenever gentle and efficient detachment of any adherent cell line is needed

Accutase performs exceptionally well in detaching cells for hESC culturing, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.

Accutase has been shown to detach a few cell lines without harm from hESCs, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

It works extremely well on embryonic and neuronal stem cells; mono layers of stem cells can be grown after passaging with Accutase. It preserves most epitopes for subsequent flow cytometry analysis. Accutase does not need to be neutralized when passaging adherent cells. The addition of more media after the cells are split dilutes Accutase so it is no longer able to detach cells. It also does not need to be aliquoted. A bottle is stable in the refrigerator for 2 months.


 

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Prices shown are current at time of publication. Where changes have occurred, prices prevailing at the time of shipment will apply. Prices are subject to change without notice.

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Our sales and customer service departments welcome quotations for laboratory set-ups and multiple-unit orders. Your inquiries are welcome and appreciated.

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If you would like to return an item, please call BioExpress customer service or contact your local representative for an RGA number. All returns must include an RGA number, include original packaging and be shipped prepaid. Items older than 30 days, sterile, or special order items are subject to review. A 15% restocking fee applies.

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BioExpress accepts no responsibility and shall not be held liable for loss, damage, expense, consequential, or accidental damage. This includes the damage to property, person, or premises resulting from these products. BioExpress MAKES NO WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING, WITHOUT LIMITATION, THE WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURCHASE. BioExpress expressly disclaims all other warranties, whether express, implied or statutory, including the warranty of MERCHANTABILITY AND FITNESS FOR USE. In no event will BioExpress be liable for consequential damages arising from the use of products described in this catalog.

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