Keep my session open?
Ending In 
The session is expired
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

HisTrap Columns, HP, GE Healthcare

Supplier: GE HEALTHCARE BIOSCIENCES

Histrap™
17524705 17524805 17-5248-01 29-0510-21 17-5248-02 17-5247-01
HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)

  • High binding capacity, at least 40 mg/mL chromatography medium
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as ÄKTA design

HisTrap HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications. HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine) 4 or (histidine) 10. The shorter (histidine) 4 will bind more weakly and the longer (histidine) 10 will bind more strongly compared with (histidine) 6. This difference in binding strength can be used during purification; since (histidine) 10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein. The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions. For convenient scaling up of histidine-tagged protein purification, use 20 mL HisPrep FF 16/10 columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.


 

ORDER

Expand All / Collapse All
Description Column Hardware Capacity Bed Dimensions Flow Rate Particle Size Supplier No. Catalog Number Unit List Price Quantity
HisTrap™ HP PP 5 mL 16 × 25 mm 5 mL/min 34 µm 17-5248-02 95056-206 Pack of 5 (5ml) Retrieving

SPECIFICATIONS

Description 95056-206
Binding Capacity At least 40 mg histidine-tagged protein/mL medium
Max. Pressure 5 bar [0.5 MPa] (70 psi)
pH Range 3 - 12
Storage 4 to 30 °C, 20% Ethanol
Enter Certificate Details

Enter the Lot number:

Choose from recent batch/lot numbers:

Or
Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected.To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty
 

Terms & Conditions

Prices:
Prices shown are current at time of publication. Where changes have occurred, prices prevailing at the time of shipment will apply. Prices are subject to change without notice.

Quotations:
Our sales and customer service departments welcome quotations for laboratory set-ups and multiple-unit orders. Your inquiries are welcome and appreciated.

Payment Terms:
Payment terms are Net 30 Days on approved credit, F.O.B. warehouse or point of manufacture. Shipping charges are prepaid and added to the invoice. C.O.D. orders will be charged a $10.00 handling fee plus shipping charges. AMEX, MasterCard, and Visa are accepted.

Minimum Order:
There is no minimum order at BioExpress. Full case quantities or minimum quantities may apply to non-stock or specialty items.

DELIVERY & SHIPPING
All orders are shipped F.O.B. warehouse or point of manufacture. Shipping charges are prepaid and added to the invoice. Orders are usually shipped within 24 hours of receipt. We ship by the most efficient and economical means available. Extra charges apply to HAZMAT and dry ice shipments.

RETURN GOODS POLICY
If you would like to return an item, please call BioExpress customer service or contact your local representative for an RGA number. All returns must include an RGA number, include original packaging and be shipped prepaid. Items older than 30 days, sterile, or special order items are subject to review. A 15% restocking fee applies.

WARRANTIES AND LIABILITIES
BioExpress accepts no responsibility and shall not be held liable for loss, damage, expense, consequential, or accidental damage. This includes the damage to property, person, or premises resulting from these products. BioExpress MAKES NO WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING, WITHOUT LIMITATION, THE WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURCHASE. BioExpress expressly disclaims all other warranties, whether express, implied or statutory, including the warranty of MERCHANTABILITY AND FITNESS FOR USE. In no event will BioExpress be liable for consequential damages arising from the use of products described in this catalog.

Print These Terms