4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride) used in nuclear staining

Supplier: Biotium

Synonyms: 2-(4-Amidinophenyl)indole-6-carboxamidine DihydrochlorideDAPI.2HCl2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride
40011
89139-054EA 105.39 USD
89139-054
4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride) used in nuclear staining
4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride)
DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Also see DAPI dilactate (catalog no. 40009), a more water soluble form of the dye.

  • Classic blue nuclear counterstain for fixed cells
  • Can be used at higher concentrations to stain live cells
  • λEx/λEm (with DNA) = 358/461 nm
  • Off-white solid soluble in H2O
  • Store at 4°C and protect from light, especially in solution

DAPI can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.


Formula: C₁₆H₁₅N₅·2HCl
MW: 350,25 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00012681
CAS Number: 28718-90-3


 

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