PowerLyzer™ PowerSoil® DNA Isolation Kit
The PowerLyzer™ PowerSoil® DNA Isolation Kit is our next generation soil DNA kit, which uses our novel, patented Inhibitor Removal Technology® to extract microbial DNA from all soil types and other environmental samples
- Ideal for High-Clay Soils, Sediments, and Low-Biomass Samples
- Utilizes 0.1 mm Glass Bead Tubes for Enhanced Homogenization of Difficult Soils, Resulting in Improved Isolation of Microbial DNA
- Optimized for Use with Bead Based Homogenizers Including the PowerLyzer™ 2
- Processes All Soil Types, Including Difficult Environmental Samples
- Patented Technology Removes 100% of Humic Substances and PCR Inhibitors
- Rapid Protocol Enables Purification of DNA from 250 mg Soil in 30 Minutes
- Applications Include Biodefense, Soil-Borne Pathogen Detection, Agriculture and Zoology Research
The PowerLyzer™ PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that removes PCR inhibitors from even the most difficult soil types, promoting successful downstream PCR analysis. When choosing between the PowerSoil® DNA Isolation Kit and the UltraClean® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.
Silica Spin Filter Tubes format. Bead Beating method. Binding capacity, Up to 20 µg per filter. 250 mg sample size. 1-24 samples. Time, 30 minutes. Bead based homogenizer required.
The isolated DNA has the highest level of purity allowing for more successful PCR and qPCR amplification of organisms from the sample. This kit contains bead tubes that are optimized for bead based homogenizers including the PowerLyzer™ 24. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds) algae, and actinomycetes (e.g. Streptomyces), and nematodes.