GeneMate α/β Gal Detection, Room Temperature Stable Enzyme Substrates
Molecular Biology Stains
CytoBLUE™ Liquid X-a-Gal, 4 mg/mL, is designed for screening of a-galactosidase detection that is often used in yeasts and other genetic models instead of β-gal
CytoBLUE, EZ-Bluez, and Firozeh are room temperature stable components of the β-gal detection systems. They have a longer shelf life than general X-Gal, IPTG, and X-a-Gal solutions. The proprietary aqueous-based solvent protects the reagents from rapid breakdown at room temperature and induces brighter colorimetric reactions. They are superior in performance, ready to use, solutions, exclusively produced and tested for all cloning and reporter assays. They are least toxic, do not require -20˚C freezing, and are stable at RT for extended periods. With these substrates there is no need to re-incubate or refrigerate to enhance color. No DMSO, No DMF, No Waiting, Shelf Life > 1 year at 40˚C.
Molecular Weight, 408.63 g/mol. Assay, Greater than 99% (HPLC). Clear Solution 2% in DMF. Biological Activity, Blue color with Lambda, pUC and M13. Elemental Analysis: Agrees with theoretical values. Thin Layer Chromatogram: Single Spot. Melting point, 230˚C.
X-α-gal (5-Bromo-4-Chloro-3-indolyl-α-D-galactopyranoside) is a chromogenic substance used to demonstrate α-galactosidase activity. Thus yeast S. cerevisiae which are positivie for this enzyme produce blue colonies when grown in the presence of X-α-gal. Used in yeast two-hybrid screens. X-α-gal can be dissolved in DMF at a concentration of 2mg/ml for premade plates or 20µg/ml for indicator plates. CytoBLUE™ is designed for screening of α galactocidase detection that is often used in yeasts and other genetic models instead of beta gal. This ready-to-use solution is economical, produces sharper contrasts and has a richer color making it ideal for auotmated colony pickers as well as manual visual selections. X-alpha-Gal: 5-Bromo-4-Chloro-3-indolyl-alpha-D-galactopyranoside.
Recommended storage condition, frozen at -20°C for 6 months. Molecular weight, 408.6. Melting point, 232-234°C. Assay, Greater than 99% (HPLC). Clear Solution 2% in DMF.
EZ-Bluez™ Liquid X-Gal, 10 mg/mL, is a premade X-Gal solution that eliminates the need to thaw or freeze the substrate. A ready-to-use solution with a concentration of 10 mg/mL is ideal for all β-gal screening assays. Firozeh™ X-Gal/IPTG Solution, 8 mg/mL, is a solution that is designed to induce as LacZ expression in the presence of (laclq) lac repressor. X-Gal Powder is a substrate most commonly used in conjunction with IPTG to identify bacterial colonies that contain recombinant plasmids by blue/white selection. X-a-Gal Powder ia a chromogenic substrate used to demonstrate a-galactosidase activity. IPTG Powder is commonly used inducer for β-Galactosidase activity in many bacterial gene sequences controlled by the lac operon. Enzyme Substrates for LacZ screening is ideal for cloning in Bacteria, Yeasts, Mammalian cells and insect cells. Perform Blue/White Assays and see the difference.