For transformed E.coli with maximum plasmid yield
LB (Miller's) Broth (25 g/L) Contents: Tryptone 10 g/L, Yeast extract 5 g/L, NaCl 10 g/L
LB (Miller's) Agar (40 g/L) Contents: Tryptone 10 g/L, Yeast extract 5 g/L, NaCl 10 g/L, Agar 15 g/L
2xYT Broth (31 g/L) Contents: Tryptone 16 g/L, Yeast extract 10 g/L, NaCl 5 g/L
LB (Lennox) Agar (35 g/L) Contents: Tryptone 10 g/L, Yeast extract 5 g/L, NaCl 5 g/L, Agar 15 g/L
SuperBroth (60 g/L) Contents: Tryptone 35 g/L, Yeast extract 20 g/L, NaCl 5 g/L
Terrific Broth (47.6 g/L) Contents: Tryptone 12 g/L, Yeast extract 24 g/L, K2HPO4 9.4 g/L, KH2PO4 2.2 g/L
Culture media has been developed specifically to bring higher performance, ease of use, and improved plasmid yield for molecular biologists. Each component has been selected and tested for optimal growth of transformed E. coli. The medias are designed to increase the plasmid density instead of the cell density, therefore, the resulting plasmid yield is much higher than other media with similar recipes. Additionally, the LB media has been buffered to maintain optimal pH for the cells as they reach the log phase of the growth curve. This ensures a consistent culture condition for the bacteria, thus further improving plasmid fidelity. These microbial culture medias are designed for ultimate convenience. The media has been packaged volumetrically to ensure that there is no waste. Each size is packed with the right amount of pre-mixed powder to produce specific volumes with no leftovers from previous batches. These medias have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins.