GeneMate Genomic Grade Bacterial Culture Media

Genomic Grade Culture Media were developed to provide molecular biologists with a reliable source of tools

LB (Miller's) Broth (25g/L) Contents: Tryptone 10.0g/L, Yeast extract 5.0g/L, NaCl 10.0g/L
2xYT Broth (31g/L) Contents: Tryptone 16.0g/L, Yeast extract 10.0g/L, NaCl 5.0g/L
LB (Lennox) Broth (20g/L) Contents: Tryptone 10.0g/L, Yeast extract 5.0g/L, NaCl 5.0g/L
NZY Broth (20.98 g/L) Contents: NZ-Amine 10.0g/L, Yeast extract 5.0g/L, NaCl 5.0g/L, MgCl2 0.98g/L
SOC Medium Contents: Tryptone 20g/L, Yeast extract 5g/L, MgCl2 10mm, MgSO4 20mm, KCl 2.5mm, NaCl 10mm, Glucose 20mm, pH 6.8
SuperBroth (60g/L) Contents: Tryptone 35.0g/L, Yeast extract 20.0g/L, NaCl 5.0g/L, pH 7.5
Terrific Broth (47.6g/L) + 8mL Glycerol Contents: Tryptone 12g/L, Yeast extract 24g/L, K2HPO4 9.4g/L, KH2PO4 2.2g/L, pH 7.2
Terrific Broth (47.6g/L) Contents: Tryptone 12g/L, Yeast extract 24g/L, K2HPO4 9.4g/L, KH2PO4 2.2g/L, pH 7.2

They minimize the unpredictability of culture, plasmid yield, and the need to repeat work. Although the recipes are standard, the difference is the quality control. We test our media with 4 different host/vector combinations. Each lot is formulated according to the recipe, the broths are sterile filtered and stead sterilized in inert polycarbonate bottles. They are tested for pH, clarity, growth, O.D. reading, pre/post culture and DNA yield/ml of culture before the product is released. We select each media component based on its ability to provide consistent growth, and maximum enrichment to the host cells. The yeast extracts and the peptone/tryptone sources are tested for consistent amino acids, minerals and salt contents. The media are also buffered to produce a consistent pH during lag/log phase. Ready2 USE is the sterile filtered and steam sterilized broth that is prepared with utmost performance guaranty. Ideal for all critical applications where consistency and reproducible quality are a requirement.