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IPTG (isopropyl-β-D-thiogalactopyranoside), Powder, GeneMate for genomic Dioxane free

Supplier: GeneMate

490002-046 490002-048
IPTG (isopropyl-β-D-thiogalactopyranoside), Powder, GeneMate for genomic Dioxane free
IPTG (isopropylthio-beta-D-thiogalactopyranoside) is an inducer of beta-galactosidase production in bacteria

IPTG is used together with the chromogenic substates X-gal or Bluo-Gal to determine lac gene expression in recombinant methods. IPTG is a chemical analog of galactose which cannot be cleaved by the enzyme ß-galactosidase. Therefore, it can serve as an inducer for activity of the E. coli lac operon by binding and inactivating the lac repressor. A stock solution, 0.1M, is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates-added after autoclaving should be approximately 0.2 mM/L. Store Frozen. Molecular weight: 238.21. White, free-flowing powder appearance. Melting point, 118°C.

Formula: C₉H₁₈O₅S
MW: 238,31 g/mol
Boiling Pt: 402 °C (1013 hPa)
Melting Pt: 109.5 to 110.5 °C
Storage Temperature: -20°C
MDL Number: MFCD00063273
CAS Number: 367-93-1
EINECS: 206-703-0



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