Molecular Biology Reagents
- Blue/White Color Screening
- Induction of Lac Operon for Protein Expression
- Genes Controlled by the Lac to Tac Promoter/Operator Sequences are Expressed to High Levels in the Presence of IPTG
Isopropyl--D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme --Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG. Features include induces E. coli lac operon activity, >99.6% by HPLC, and available as powder and stabilized stock solution. IPTG powder can be stored for 12 months at -20°C. Shipped on dry ice or blue ice.