IPTG (isopropyl-β-D-thiogalactopyranoside), ultrapure
IPTG, a non-metabolizable galactose analog, is commonly used in cloning procedures that require induction of β-galactosidase activity
IPTG powder can be stored at Room Temperature. For optimal shelf-life, we recommend storage, with dessicant, at -20oC. IPTG stock solution is typically made at a 1M concentration and stored at -20oC. We recommend making single-use aliquots to prevent repeated freeze-thaw cycles of IPTG in solution.
IPTG is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. Teknova IPTG is Dioxane-Free