IPTG (isopropyl-β-D-thiogalactopyranoside), ultrapure


I3301 I3325 I3305
100219-816 100218-372 CA100218-372 CA100219-244 100219-244 CA100219-816
IPTG (isopropyl-β-D-thiogalactopyranoside), ultrapure
IPTG (Isopropyl-β-D-thiogalactopyranoside)
IPTG, a non-metabolizable galactose analog, is commonly used in cloning procedures that require induction of β-galactosidase activity

IPTG powder can be stored at Room Temperature. For optimal shelf-life, we recommend storage, with dessicant, at -20oC. IPTG stock solution is typically made at a 1M concentration and stored at -20oC. We recommend making single-use aliquots to prevent repeated freeze-thaw cycles of IPTG in solution.

IPTG is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. Teknova IPTG is Dioxane-Free


Formula: C₉H₁₈O₅S
MW: 238.31 g/mol
MDL Number: MFCD00063273
CAS Number: 367-93-1

 

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