Fast-Ion Midi Plasmdi Kits, IBI Scientific
IBI’s Midi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures
The plasmid DNA is suitable for transfection, sequencing reactions, PCR, microinjection, restriction digestion, and in-vitro transcription.
In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA/RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate binds to the column. The contaminants are washed away with a wash buffer. The purified plasmid DNA is eluted by a high salt buffer and precipitated with isopropanol. The entire procedure can be completed in less than 2 hours.