Isol-RNA Lysis Reagent, 5PRIME
- Easy-to-follow protocol for lysis and homogenization
- Optimized lysis conditions to purify RNA
- High yields of RNA even from difficult tissues
- Compatibility with a variety of tissue types
The phenol/guanidine-based Isol-RNA Lysis Reagent™ is optimized for lysis of a variety of tissues types before RNA isolation with standard homogenization methods and alcohol precipitation. The combination of tissue lysis with Isol-RNA Lysis Reagent and organic extraction aided by Phase Lock Gel™ is the method of choice for RNA purification from tissues with high fat content like brain, breast or adipose tissue. The combination of organic extraction and chaotropic disruption contributes to efficient lysis for high yields of total RNA.
Further 5 PRIME Isol-RNA Lysis Reagent, like TRIzol, allows for the efficient lysis of a variety of biological samples for the simultaneous isolation of RNA, DNA and protein (please see additional protocols section for further information).
The standard Isol-RNA Lysis Reagent protocol provides an easy-to-follow procedure, combining Isol-RNA-lysis with standard homogenization methods and ethanol precipitation. Tissue samples are homogenized in Isol-RNA Lysis Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. RNA partitions to the upper, aqueous phase while DNA partitions to the interphase and proteins to the lower, organic phase. RNA is precipitated from the aqueous phase by addition of isopropanol. The pellet is then washed with ethanol and redissolved in RNase-free water. For high performance in downstream applications, subsequent purification of the RNA is recommended, using kits, which are based on silica-membrane technology, in order to remove any contaminating phenol. Alternatively, the RNA can be cleaned up by an ethanol precipitation.
Identical Performance as Leading Brand
5 PRIME Isol-RNA Lysis Reagent (5P) is as effective for RNA isolation as other lysis reagents based on the Chomczynski* protocol. Mouse RNA was isolated from the organs indicated above using 5 PRIME Isol-RNA Lysis Reagent (5P) or products from brand I or A. All three methods yielded the same amount and quality of RNA. The figure shows a virtual gel image generated after analysis with the Agilent Bioanalyzer.