, 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
Ponceau S solution 0.1% in 1% acetic acid, AdvanStain™ protein stain
Protein stain for membranes.
- Quickly detects proteins in 5 minutes or less
- Reversible - destaining can be done in minutes
- Compatible with downstream Western blotting after destaining
AdvanStain Ponceau rapidly detects proteins on nitrocellulose and PVDF membranes, allowing you to check the quality of protein transfer before proceeding to Western blotting.
After transferring protein from a gel to a PVDF or nitrocellulose membrane, incubate the membrane in the ready-to-use AdvanStain Ponceau solution for 5 minutes or less to detect protein bands. The staining allows you to quickly check that transfer was even across the gel, and determine whether there are any transfer artifacts such as bubbles, before you move on to incubate the blot with primary antibody. The membrane can be detained in minutes by incubating in water, and is then ready for Western blotting.