Collagenase, Type IV from Clostridium histolyticum, >160 u/mg, MP Biomedical
Collagenases degrade native helical collagen fibrils
- Presentation: Brown Flakey Powder
- Collagenase Type IV has low tryptic activity
- Commonly used for islets and other applications where receptor integrity is crucial
Collagenase is used for tissue dissociation combined with other enzymes such as elastase, trypsin, and/or papain.
The material has been tested for Caseinase, Clostripain and Tryptic activities.
Activators: Ca2+ is required.
Inhibitors: Metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by a2-macroglobulin - a large plasma glycoprotein. The human skin enzyme is inhibited by human serum but granulocyte collagenase is not. Human serum contains a2-macroglobulin and a1-antitrypsin that may inhibit certain collagenases.
It has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes.
Collagenase Type IV has low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial.