Ampicillin is used as a selectable marker in many commonly used plasmids. A functional Ampicillin gene will confer resistance to cells containing the plasmid.
- Inhibits cell wall synthesis by inhibiting formation of the peptidoglycan cross-linkages
- Acts as a competitive inhibitor of transpeptidase
Inhibits bacterial cell wall synthesis by binding to one or more of the penicillin binding proteins (PBPs) that inhibit the final transpeptidation step of peptidoglycan synthesis in bacterial cell walls, thus inhibiting cell wall biosynthesis.