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CELLine Bioreactors, Argos Technologies

Supplier: ARGOS TECHNOLOGIES INC.

CELLine Bioreactor Flasks are ideal for monoclonal antibodies and expressed recombinant cellular protein production. Viable in vitro alternative to using mouse ascites, standard cell culture, or tissue culture products. Bioreactors utilize innovative membrane technology to separate the cell cultivation area from the media chamber. Cells and cellular-secreted products with a MWCO over 10kD are retained between two non-cytotoxic membranes. The upper semi-permeable membrane allows continuous nutrient diffusion and waste elimination. The lower membrane provides direct oxygenation and ensures optimum gas exchange. Separate ports give access to either media or cell cultivation chamber allowing easy change of expended medium without interference of cell growth, function, or gas exchange.

All bioreactors feature polystyrene housing, molded silicone gas transfer bottom, a 10,000 MWCO regenerated cellulose semi-permeable membrane, and a 0.2µm bottom vent membrane barrier located in the cell innoculum port. Bioreactors are stackable to save space in an incubator. Gamma irradiation sterilized, and pressure tested. Can be reused with same cell type under aseptic conditions.

The CL1000 is ideal for monoclonal antibody production up to 5mg/mL, recombinant protein expression, and concentrated cell densities of 108 cell/mL. Allows production of up to 1 x 109 cells per cell compartment. The 15mL cultivation chamber is ideal for small production runs. A 1000mL media compartment has vertical and horizontal volume markings from 100–1000mL. Produces ~80mg/month of antibody.

The CL350 is designed for stem cell expansion, primary cell culture, and antibody production. Allows production of up to 2 x 108 cells per cell compartment. The 5mL cultivation chamber is ideal for all tissues where cell density is important. A 350mL media compartment has vertical and horizontal volume markings from 50–350mL. Produces ~27mg/month of antibody.

CELLine Adhere is developed for the expression of biomolecules in anchorage dependent cell lines. The observed product concentrations in the cell compartment are in the range of 1–5mg/mL and are often several hundred times higher than what is obtained in either roller bottles or tissue culture flasks with the same cell type. Ideal for use with CHO, BHK, HEP2, or any other adherent cell line, CELLine Adhere is available in a one liter format. The flask is reusable and greatly reduces the downstream processing cost of concentration and purification. Serum usage is also reduced as a result of the compartmentalized chambers in the flask. The result is a low cost bioreactor for the production of expressed biomolecules.

Ordering information: Available in sterile, individually wrapped bags.

 

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Description Supplier No. Catalog Number Unit List Price Quantity
CELLine CL1000 90005 90005 Case of 3 Retrieving
CELLine Adhere 900 25 90025 Case of 3 Retrieving
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