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GelRed™ and GelGreen™ Nucleic Acid Gel Stains, Biotium

Supplier: BIOTIUM, INC

GelRed™ and GelGreen™ are a new generation of fluorescent nucleic acid gel stains designed to replace the highly toxic ethidium bromide (EtBr). GelRed and GelGreen are superior to EtBr and other EtBr alternatives by having a combination of low toxicity, high sensitivity and exceptional stability.

GelRed™ and GelGreen™ use a novel yet very simple concept to reduce genotoxicity by preventing the dyes from entering living cells. A DNA-binding dye can be made nonmutagenic or substantially less mutagenic by denying contact with genomic DNA in living cells. Thus, GelRed™ and GelGreen™ are engineered such that the chemical structures of the dyes are incapable of crossing cell membranes. The Ames test confirmed that GelRed™ and GelGreen™ are nonmutagenic at concentrations well above their working concentrations used for gel staining. Furthermore, environmental safety tests showed that GelRed™ and GelGreen™ are nonhazardous and nontoxic to aquatic life. As a result, GelRed™ and GelGreen™ can be disposed of down the drain or in regular trash.

GelRed™ and GelGreen™ are highly sensitive either as precast gel stains or post gel stains. Designed primarily for use with a 312/302 nm UV transilluminator, GelRed is much more sensitive than EtBr. GelRed™ can also be used as a highly sensitive precast gel stain. GelGreen™ is developed to meet the needs of researchers who use a 488 nm laser-based gel scanner or a Dark Reader that uses a visible blue light for excitation. The dyes are stable in water at room temperature for long-term storage, and can be microwaved for making precast gels. Both dyes are also very photostable allowing use under normal room lighting.

 

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GelRed™ Nucleic Acid Gel Stains
Description Size Concentration Supplier No. Catalog Number Unit List Price Quantity
GelRed™ Nucleic Acid Gel Stain 500 µL 10,000X in water 41003 89139-138 Each (500µl) Retrieving Restricted
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