DAB tetrahydrochloride hydrate
, DAB HCl hydrate
, 3,3',4,4'-Biphenyltetramine tetrahydrochloride hydrate
3,3'-Diaminobenzidine tetrahydrochloride hydrate ≥98%, Reagent Grade for biochemistry
3,3'-Diaminobenzidine tetrahydrochloride hydrate
Commonly used for detection of horseradish peroxidase in membrane-bound and in situ applications. Insoluble end product is resistant to alcohol and fading, making it ideal for immunohistochemistry.
Insoluble.Reaction Condition: 6 mg in 10 ml of 50 mM Tris, 0.1 M NaCl, pH 7.6 and 0.01% H₂O₂.The presence of Nickel or Cobalt increases sensitivity.