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HEPES, Free Acid, High Purity

Supplier: VWR

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acidHEPES free acid
0511-250G 0511-1KG 0511-50G
One of the best general-purpose buffers for biological research. Excellent for tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, and CO2 fixation.

Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates. Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.

Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Melting Pt: 234°C
Density: 1.448 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
EINECS: 230-907-9
Merck Index: 13,04672
Specification Test Results
DNase none detected
Heavy Metals (as Pb) <= 0.0005 %
Iron <= 0.0005 %
pKa @20°C 7.45 - 7.65
Protease none detected
Purity (Dry Basis) > 99 %
Residue on Ignition < 0.2 %
RNase none detected



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Size Packaging Application Supplier No. Catalog Number Unit List Price Quantity
50 g HDPE Bottle - VWRV0511-50G 0511.50G Each Retrieving Restricted
250 g HDPE Bottle - VWRV0511-250G 0511.250G Each Retrieving Restricted
1 kg HDPE Bottle - VWRV0511-1KG 0511.1KG Each Retrieving Restricted
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