VWR Life Science SOB Broth, Biotechnology Grade
Media for Microbiology
Dehydrated Media for Microbiology
SOB broth powder is used for the preparation of nutritionally rich growth medium and transformation of competent <italic>E
SOB Broth (Super Optimal Broth) growth medium is nutritionally rich, containing casein hydrolysate and yeast extract as sources of nitrogenous compounds and growth factors,and magnesium sulfate as a supply of magnesium ions necessary for DNA replication.
when dissolved at 25.5 g per liter of water,yields the formulation
Tryptone -------- 20 Grams/L
Yeast Extract- - -5 Grams/L
Sodium Chloride--0.5 Gram/L
Medium should be autoclaved or sterile-filtered. Allow the solution to cool to 50 °C or below before adding supplemental components and antibiotic. Add 20 mL of 1 M MgSO4.Store SOB Broth powder at room temperature (18 – 26 °C). Store prepared medium cold (2 – 8 °C).