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VWR Life Science AMRESCO Superbroth, Powder

Supplier: VWR

Superbroth powder is rich medium used for obtaining high yields of lambda phage in liquid lysates.

  • Formulation in grams/L
  • Tryptone----------32
  • Yeast Extract-----20
  • Sodium Chloride--5
  • Final pH------------7.2 (± 0.2 @ 25°C)
Superbroth medium contains high levels of tryptone and yeast extract, which provide essential nutrients and growth factors to support high density growth of E. coli. This rich medium is often used to obtain high yields of lambda bacteriophage phagemids. The Superbroth formulation also contains sodium chloride to help maintain an isotonic environment during the bacterial growth phase.

Mix 995 mL purified water with 5 mL of 1 N sodium hydroxide.Suspend 57 g of Superbroth, Powder in 1 L of the water/sodium hydroxide solution.Allow the powder to dissolve with gentle stirring and heating, if necessary.Autoclave for 15 minutes at 15 psi at 121°C. Note: Alternatively, medium may be sterile-filtered.Allow the solution to cool to 50°C or below before adding supplemental components and antibiotic.

Store Superbroth, Powder at room temperature (18 – 26°C). Store prepared medium cold (2 –8°C).


 

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Description Size Supplier No. Catalog Number Unit List Price Quantity
SUPERBROTH, POWDER 500 g J868-500G J868.500G Each Retrieving Restricted
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