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VWR Life Science AMRESCO M9 Medium Broth, Powder

Supplier: AMRESCO INC

M9 Medium Broth powder is a minimal medium, based on the M9 Minimal Salts, which supports the growth of Escherichia coli.Can maintain bacteria for use with M13 phage.

  • Save time using premixed and ready-to-use media
  • Homogenous and high quality powder
  • Media for lambda propagation.

This medium is useful in maintaining positive selection pressure on plasmids coding for substances, such as amino acids or vitamins. It can also be used to maintain bacteria for use with M13. M9 Broth can be supplemented with specific amino acids or other metabolites that allows for specific selection pressures that the researcher may require.

Suspend 10.5 g of M9 Medium Broth, Powder in 1 L of distilled, deionized water.Allow the powder to dissolve with gentle stirring and heating, if necessary.Adjust the pH to 7.4 with sodium hydroxide.Autoclave for 15 minutes at 15 psi at 121°C. Note: Alternatively, medium may be sterile-filtered.Allow the solution to cool to 50°C or below before adding supplemental components and antibiotic.Add 2 mL of sterile 1 M magnesium sulfate, 10 mL of sterile 20% glucose and 0.1 mL of sterile 1 M calcium chloride.

1L of media contains 6 grams of sodium phosphate dibasic, 3 grams of potassium phosphate monobasic, 0.5 grams of sodium chloride and 1 gram of ammonium chloride.

Store M9 Powders at room temperature (18 to 26°C). Store prepared media cold (2 to 8°C).

 

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Description Size Supplier No. Catalog Number Unit List Price Quantity
M9 MEDIUM BROTH, POWDER 500 g J863-500G J863.500G Each Retrieving Restricted
M9 MEDIUM BROTH, POWDER 100 g J863-100G J863.100G Each Retrieving Restricted
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