Ethidium bromide for biotechnology

Synonyms: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
0492-25G 0492-5G
97064-600 97064-602
Ethidium bromide for biotechnology
Ethidium bromide
At a concentration of 0.5μg/ml, ethidium bromide is useful in agarose or acrylamide gels for band detection. It is used to detect as little as 10ng of nucleic acid. At higher concentrations (0.5–1.0μg/ml), it may be used to facilitate purification of DNA in a cesium chloride gradient.

  • Fluorescent stain for visualizing nucleic acids

For maximum convenience, VWR offers ethidium bromide in various forms, including ready-to-use solutions and powdered forms. Each is packaged in shatter-resistant packaging for added safety. VWR’s ethidium bromide dropper bottle offers the ultimate convenience and safety. Simply dispense one drop of ethidium bromide into 50ml of agarose gel or running buffer (TBE or TAE) or staining solution.

Formula: C₂₁H₂₀BrN₃
MW: 394.31 g/mol
Boiling Pt: 72 °C (0,1 mmHg)
Melting Pt: 261…264 °C
Density: 1.01 g/cm³ (20 °C)
Flash Pt: <100 °C
Storage Temperature: Ambient
MDL Number: MFCD00011724
CAS Number: 1239-45-8
EINECS: 214-984-6
UN: 2811
ADR: 6.1,I
Merck Index: 13,04751
Specification Test Results
DNase none detected
Loss on Drying = 5 %
Protease none detected
Purity (Dry Basis) = 98 %
RNase none