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Phenol:Chloroform, Low pH, Biotechnology Grade


  • Manufactured Using Exceptionally High Purity Phenol that is Double Distilled & Packaged Under Nitrogen
  • Liquid Phenols Offer a Safer, Easier Alternative to Crystalline Phenol
  • Optimized pH for the Purification of RNA
  • Long Term Stability > One Year

For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. AMRESCO’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.

UN: 2810
ADR: 6.1,II
Specification Test Results
O.D.@ 330nm < = 0.2
O.D.@ 405nm <= 0.1
O.D.@ 510nm <= 0.1
pH @25°C 4.9 - 5.5
Purity >= 99 %



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Size Application Description Supplier No. Catalog Number Unit List Price Quantity
100 mL A 1:1 mixture useful for preparing RNA with maximal recovery of poly (A+) RNA for subsequent mRNA purification and generation of cDNA. PHENOL:CHLOROFORM, LOW pH 0966-100ML 0966.100ML Each (100ml) Retrieving Restricted
400 mL A 1:1 mixture useful for preparing RNA with maximal recovery of poly (A+) RNA for subsequent mRNA purification and generation of cDNA. PHENOL:CHLOROFORM, LOW pH 0966-400ML 0966.400ML Each (400ml) Retrieving Restricted
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