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Protein dotMETRIC™ Assay, G-Biosciences

Supplier: G-BIOSCIENCES

Rapidly mix 1µL protein sample with the supplied dilution buffer and apply 1µL of the solution to the test strip by point-of-contact capillary action. Under the assay’s specific buffer conditions the protein enters into the matrix of the test strip, binds and saturates as protein then diffuses in a circular manner. Develop the test strip in approximately five minutes. A circular protein spot is produced. By measuring the diameter of the spots with the Protein dotMETRIC™ scale you can easily determine concentration of protein. No expensive spectrophotometers or cuvettes required.

For increased reproducibility and test reliability, the dotMETRIC™ kits are offered with an optional Spot Application Device. The Spot Application Device allows application of samples using fixed volume (1µL) capillary tips and simplifies the task of applying the protein solution on the test strips by point-of-contact capillary action. The Spot Application Device simplifies the application of one or more samples and improves the reliability of results. The dotMETRIC™ assay is able to resist common laboratory agents such as Triton® X-100, Triton® X-114, Thesit®, Tween® 20, Nonidet® P-40, SDS, reducing agents such as ß-mercaptoethanol and DTT, sugars, cobalt, EDTA, Tris buffers, and so forth.

For rapid estimation of protein concentration in cellular fractions, tissue and cell lysates, and chromatography purification fractions.

Ordering information: Accessories available.

 

ORDER

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Description Size Supplier No. Catalog Number Unit List Price Quantity
Protein dotMETRIC™ 300 Preps 786-20 P-7860-1 Each Retrieving Restricted
Protein dotMETRIC™ kit with Spot Application Device & Capillary Tips 300 Preps 786-21 P-7860-2 Each Retrieving Restricted
restricted You must log in to order restricted items. We request that you provide the required business documentation to purchase this product for the first time.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email info@bioexpress.com or fax 800-574-7892 referencing your BioExpress account number. Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
BioExpress will not lift restrictions for residential shipping addresses.

ACCESSORIES

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Accessories
Description Supplier No. Catalog Number Unit List Price Quantity
Capillary Application Tips, Glass, 1 µL 786-23 P-7860-4 Pack of 100 Retrieving
Developing Trays 786-24 P-7860-5 Pack of 2 Retrieving Restricted
dotMETRIC™ Application Device 786-63 P-7870-4 Each Retrieving Restricted
Sample Application Tips 786-64 P-7870-5 Pack of 96 Retrieving Restricted
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