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Cell Viability and Proliferation Assays, Biotium

Supplier: Biotium

30007 30006 30025-2 30002-T 30020-1 30025-1 30002 30020-2 30026
89138-908 89138-906 89138-952 89260-208 89138-938 89138-950 89138-886 89138-940 89138-954
Cell Viability and Proliferation Assays, Biotium
Biotium offers cell proliferation assays to quantitate live or dead cells by absorbance, fluorescence, or luminescence.

  • Aassay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells

Calcein-AM and ViaFluorâ„¢ CFSE are initially colorless compounds that are hydrolyzed by cellular esterases to label the cytoplasm with green fluorescence. Calcein-AM and ViaFluorâ„¢ CFSE can be used to quantitate viable cells by fluorescence microplate reader. ViaFluorâ„¢ CFSE stably labels cells and also can be used to track cell divisions over multiple generations by flow cytometry analysis in the FITC channel. The Cytotoxicity and Viability Assay pairs calcein-AM with the red fluorescent vital dye ethidium homodimer III for quantitation of live and dead cells.

The ATP-Glo cell viability assay uses Firefly luciferase lumenescence to measure cellular ATP levels. ATP-Glo is highly sensitive, and can detect as little as one cell or 0.01 picomole of ATP, with signal linearity for ATP detection within 6 orders of magnitude. ATP-Glo is a flash-type luminescence assay (signal is stable for ~ 1 minute), for use with a single read luminometer or luminescence plate reader with sample injector.

MTT and XTT are reduced by viable cells to form colored formazin salts, which can be measured by absorbance. MTT requires cell lysis prior to measurement, while XTT does not. Resazurin is reduced by viable cells from a dark blue colored compound to a red fluorescent compound, which can be measured by fluroescence or absorbance.

Ordering information: Kit size based on 96-well assay. CFDA-SE assay number varies by application.



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Prices shown are current at time of publication. Where changes have occurred, prices prevailing at the time of shipment will apply. Prices are subject to change without notice.

Our sales and customer service departments welcome quotations for laboratory set-ups and multiple-unit orders. Your inquiries are welcome and appreciated.

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Payment terms are Net 30 Days on approved credit, F.O.B. warehouse or point of manufacture. Shipping charges are prepaid and added to the invoice. C.O.D. orders will be charged a $10.00 handling fee plus shipping charges. AMEX, MasterCard, and Visa are accepted.

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There is no minimum order at BioExpress. Full case quantities or minimum quantities may apply to non-stock or specialty items.

All orders are shipped F.O.B. warehouse or point of manufacture. Shipping charges are prepaid and added to the invoice. Orders are usually shipped within 24 hours of receipt. We ship by the most efficient and economical means available. Extra charges apply to HAZMAT and dry ice shipments.

If you would like to return an item, please call BioExpress customer service or contact your local representative for an RGA number. All returns must include an RGA number, include original packaging and be shipped prepaid. Items older than 30 days, sterile, or special order items are subject to review. A 15% restocking fee applies.

BioExpress accepts no responsibility and shall not be held liable for loss, damage, expense, consequential, or accidental damage. This includes the damage to property, person, or premises resulting from these products. BioExpress MAKES NO WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING, WITHOUT LIMITATION, THE WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURCHASE. BioExpress expressly disclaims all other warranties, whether express, implied or statutory, including the warranty of MERCHANTABILITY AND FITNESS FOR USE. In no event will BioExpress be liable for consequential damages arising from the use of products described in this catalog.

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